CR Name:
Species:    Tissue or Cell: 

Chromatin Regulator

Alias

KDM5BCT31; PLU1; PUT1; PLU-1; JARID1B; RBBP2H1A

External Links:

Wiki    GeneCards    NCBI    UniProt

Related histone modifications:

H3K4me3;H3K4me2

Introduction

Full name: Lysine (K)-specific demethylase 5B . KDM5B displays a demethylase-containing Jmjc domain, along with PHD domains and an ARID domain. It is highly expressed in breast cancer cells, while its expression is limited in normal tissues, requiring iron and α-ketoglutarate to function. Additionally, KDM5B is involved in processes such as development, differentiation, cell proliferation, transcriptional regulation, and chromatin remodeling (1-5). KDM5B can remove mono-, di- and trimethylation on histone H3K4 (1).

Function and Interaction

KDM5B regulates the expression of many genes related to development, the cell cycle, signal transduction, and metabolism (3,6-7). KDM5B overexpression can increase the transcription of the androgen receptor, which plays a vital role in prostate cancer development via its JmjC domain (1). KDM5B can cooperate with TFAP2C and Myc in suppressing p21 (cip) (CDKN1A), which acts as an inhibitor during the cell cycle (6). This factor can be recruited by Rb to the promoter of some E2f target genes, enabling it to take part in the Rb pathway (8). KDM5B is an activator, rather than a repressor in the self-renewal network (9). It also plays a role in cell-fate decisions (10). However, KDM5B also acts as a transcriptional repressor, and its interactions with brain factor-1 (BF-1) and paired box 9 (PAX9) can dramatically increase its suppression effect (11-12). KDM5B interacts with class I HDACs, class IIa HDACs and N-CoR in breast cancer cells (4). KDM5B can also interact with TIEG1 (transforming growth factor-β inducible early gene-1) to inhibit tumorigenesis by regulating TGF-β signaling (13).

Disease Association

KDM5B is upregulated in breast cancer and is involved in regulating cell proliferation by interacting with tumor suppressor genes. KDM5Bis also upregulated in prostate cancer cells (1-2,5,12).

ChIP-Seq data


SpeciesCell lineCell typeTissueDataDownloadSend to CistromeAnalysis FiguresComparisonReference
Homo sapiensMCF-7EpitheliumMammary GlandGSE46073 ,GSM1122652
Click  DownloadClick24937458

ChIP-Seq data of related histone modifications


Products/SubstratesSpeciesCell lineCell typeTissueDataDownload Send to CistromeAnalysis FiguresReference
H3K4me3 Homo sapiens H1 Embryonic Stem Cell Embryo GSE41009 ,GSM1006722 Bed     Big wiggle
Click   Download23382218
H3K4me2 Homo sapiens MCF-7 Epithelium Mammary Gland GSE46073 ,GSM1122654 Bed     Big wiggle
Click   Download24937458
H3K4me2 Homo sapiens MCF-7 Epithelium Mammary Gland GSE46073 ,GSM1122655 Bed     Big wiggle
Click   Download24937458
H3K4me3 Homo sapiens MCF-7 Epithelium Mammary Gland GSE46073 ,GSM1122656 Bed     Big wiggle
Click   Download24937458
H3K4me2 Homo sapiens SUM159PT Epithelium Mammary Gland GSE46073 ,GSM1122665 Bed     Big wiggle
Click   Download24937458
H3K4me3 Homo sapiens SUM159PT Epithelium Mammary Gland GSE46073 ,GSM1122666 Bed     Big wiggle
Click   Download24937458
H3K4me3 Homo sapiens MDA-MB-231 Epithelium Mammary Gland GSE49651 ,GSM1204472 Bed     Big wiggle
Click   Download24885402
H3K4me3 Homo sapiens MDA-MB-231 Epithelium Mammary Gland GSE49651 ,GSM1204473 Bed     Big wiggle
Click   Download24885402
H3K4me3 Mus musculus R1 Embryonic Stem Cell Embryo GSE53087 ,GSM1282137 Bed     Big wiggle
Click   Download24495580
H3K4me3 Mus musculus R1 Embryonic Stem Cell Embryo GSE53087 ,GSM1282139 Bed     Big wiggle
Click   Download24495580
H3K4me2 Homo sapiens HCC2157 Epithelium Mammary Gland GSE46055 ,GSM1402462 Bed     Big wiggle
Click   Download24937458
H3K4me3 Homo sapiens HCC2157 Epithelium Mammary Gland GSE46055 ,GSM1402463 Bed     Big wiggle
Click   Download24937458
H3K4me3 Homo sapiens MCF-7 Epithelium Mammary Gland GSE59980 ,GSM1462965 Bed     Big wiggle
Click   Download25122745
H3K4me3 Mus musculus R1 Embryonic Stem Cell Embryo GSE74268 ,GSM1915970 Bed     Big wiggle
Click   Download26628093
H3K4me2 Homo sapiens MDA-MB-231 Epithelium Mammary Gland GSE17001 ,GSM425484 Bed     Big wiggle
Click   Download19581485
H3K4me3 Homo sapiens H1 Embryonic Stem Cell Embryo GSE16256 ,GSM605315 Bed     Big wiggle
Click   Download19829295
H3K4me3 Homo sapiens K562 Erythroblast Bone Marrow GSE28162 ,GSM608165 Bed     Big wiggle
Click   Download21596310
H3K4me2 Homo sapiens MCF-7 Epithelium Mammary Gland GSE31151 ,GSM771029 Bed     Big wiggle
Click   Download23001124
H3K4me3 Homo sapiens K562 Erythroblast Bone Marrow GSE35294 ,GSM865280 Bed     Big wiggle
Click   Download23754963
H3K4me3 Homo sapiens K562 Erythroblast Bone Marrow GSE35294 ,GSM865281 Bed     Big wiggle
Click   Download23754963
H3K4me3 Homo sapiens H1 Embryonic Stem Cell Embryo GSE35375 ,GSM867107 Bed     Big wiggle
Click   Download22374078
H3K4me3 Homo sapiens H1 Embryonic Stem Cell Embryo GSE35375 ,GSM867108 Bed     Big wiggle
Click   Download22374078
H3K4me3 Mus musculus R1 Embryonic Stem Cell Embryo GSE36294 ,GSM886518 Bed     Big wiggle
Click   Download24381111
H3K4me3 Homo sapiens H1 Embryonic Stem Cell Embryo GSE16256 ,GSM906405 Bed     Big wiggle
Click   Download19829295
H3K4me3 Homo sapiens MCF-7 Epithelium Mammary Gland GSE35583 ,GSM945269 Bed     Big wiggle
Click   Download22955617

Notice: For bigwiggle or bed file download, if the browser cannot automatically download the file, users can right click the hyperlink and then click the "Save link as..." button, which will automatically redirect to download dialogue box, or click the "Copy link address" button and access the website in a new tab page. For data analysis in Cistrome, users should first login into the Cistrome website, then press the "Send Bed" or "Send Big wiggle" button. For data download by Cistrome, users can follow this tutorial.

References

1. Xiang, Y., Zhu, Z., Han, G., Ye, X., Xu, B., Peng, Z., Ma, Y., Yu, Y., Lin, H., Chen, A.P. et al. (2007) JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer. P Natl Acad Sci USA, 104, 19226-19231.
2. Lu, P.J., Sundquist, K., Baeckstrom, D., Poulsom, R., Hanby, A., Meier-Ewert, S., Jones, T., Mitchell, M., Pitha-Rowe, P., Freemont, P. et al. (1999) A novel gene (PLU-1) containing highly conserved putative DNA chromatin binding motifs is specifically up-regulated in breast cancer. J Biol Chem, 274, 15633-15645.
3. Scibetta, A.G., Santangelo, S., Coleman, J., Hall, D., Chaplin, T., Copier, J., Catchpole, S., Burchell, J. and Taylor-Papadimitriou, J. (2007) Functional analysis of the transcription repressor PLU-1/JARID1B. Mol Cell Biol, 27, 7220-7235.
4. Barrett, A., Santangelo, S., Tan, K., Catchpole, S., Roberts, K., Spencer-Dene, B., Hall, D., Scibetta, A., Burchell, J., Verdin, E. et al. (2007) Breast cancer associated transcriptional repressor PLU-1/JARID1B interacts directly with histone deacetylases. Int J Cancer, 121, 265-275.
5. Catchpole, S., Spencer-Dene, B., Hall, D., Santangelo, S., Rosewell, I., Guenatri, M., Beatson, R., Scibetta, A.G., Burchell, J.M. and Taylor-Papadimitriou, J. (2011) PLU-1/JARID1B/KDM5B is required for embryonic survival and contributes to cell proliferation in the mammary gland and in ER+ breast cancer cells. International Journal of Oncology, 38, 1267-1277.
6. Wong, P.P., Miranda, F., Chan, K.Y.V., Berlato, C., Hurst, H.C. and Scibetta, A.G. (2012) Histone Demethylase KDM5B Collaborates with TFAP2C and Myc To Repress the Cell Cycle Inhibitor p21(cip) (CDKN1A). Mol Cell Biol, 32, 1633-1644.
7. Schmitz, S.U., Albert, M., Malatesta, M., Morey, L., Johansen, J.V., Bak, M., Tommerup, N., Abarrategui, I. and Helin, K. (2011) Jarid1b targets genes regulating development and is involved in neural differentiation. Embo Journal, 30, 4586-4600.
8. Nijwening, J.H., Geutjes, E.J., Bernards, R. and Beijersbergen, R.L. (2011) The Histone Demethylase Jarid1b (Kdm5b) Is a Novel Component of the Rb Pathway and Associates with E2f-Target Genes in MEFs during Senescence. PLoS One, 6.
9. Xie, L.Q., Pelz, C., Wang, W.S., Bashar, A., Varlamova, O., Shadle, S. and Impey, S. (2011) KDM5B regulates embryonic stem cell self-renewal and represses cryptic intragenic transcription. Embo Journal, 30, 1473-1484.
10. Dey, B.K., Stalker, L., Schnerch, A., Bhatia, M., Taylor-Papidimitriou, J. and Wynder, C. (2008) The histone demethylase KDM5b/JARID1b plays a role in cell fate decisions by blocking terminal differentiation. Mol Cell Biol, 28, 5312-5327.
11. Tan, K., Shaw, A.L., Madsen, B., Jensen, K., Taylor-Papadimitriou, J. and Freemont, P.S. (2003) Human PLU-1 has transcriptional repression properties and interacts with the developmental transcription factors BF-1 and PAX9. J Biol Chem, 278, 20507-20513.
12. Yamane, K., Tateishi, K., Klose, R.J., Fang, J., Fabrizio, L.A., Erdjument-Bromage, H., Taylor-Papadimitriou, J., Tempst, P. and Zhang, Y. (2007) PLU-1 is an H3K4 dernethylase involved in transcriptional repression and breast cancer cell proliferation. Mol Cell, 25, 801-812.
13. Kim, J., Shin, S., Subramaniam, M., Bruinsma, E., Kim, T.D., Hawse, J.R., Spelsberg, T.C. and Janknecht, R. (2010) Histone demethylase JARID1B/KDM5B is a corepressor of TIEG1/KLF10. Biochem Bioph Res Co, 401, 412-416.

Figure Gallery

pix pix pix pix pix pix

About Chromatin Regulator

Chromatin Regulator Cistrome
is a unique database integrating curated information of CRs, CR ChIP-seq datasets, CR related HM ChIP-seq datasets, and analysis of the relationship between CRs and HMs ChIP-seq pairs in human and mouse.

Contact

houshuang [at] tongji dot edu dot cn