Chromatin Regulator | Alias | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| EHMT2 | C6orf30;KMT1C;BAT8;NG36;G9A | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
External Links: | Wiki GeneCards NCBI UniProt | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Related histone modifications: | H3K9me1;H3K9me2;H3K27me1;H3K27me2;H3K27me3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Introduction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Full name: Euchromatic Histone Lysine Methyltransferase 2. EHMT2 is a ubiquitously expressed SET domain-containing protein that was originally characterized as a molecule encoded by a gene mapped in the class III region of the human major histocompatibility complex locus(1).EHMT2 contains a SET domain which can bind effectively to unmodified and H3K9me1 peptides,an ankyrin repeats which binds with strong preference to N-terminal H3 peptides containing mono- or dimethyl K9(2,3).EHMT2 is a histone methyltransferase that specifically mono- and dimethylate lysine 9 of histone H3(H3K9me1 and H3K9me2) in euchromatin,mediates monomethylation of lysine 56 of histone(H3K56me1) in G1 phase,weakly methylates lysine 27 of histone H3(2-5). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Function and Interaction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| EHMT2 promotes interaction between histone H3 and PCNA and regulates DNA replication by mediates monomethylation of lysine 56 of histone H3(H3K56me1) in G1 phase(4).EHMT2 methylates DNA ligase 1 (LIG1) which recruits UHRF1 to DNA replication sites and is required for DNA methylation maintenance(6).EHMT2 regulates breast cancer growth by modulating iron homeostasis through the repression of ferroxidase hephaestin(7).EHMT2 has a dominant function in transcriptional repression of K(+) channels and in acute-to-chronic pain transition after nerve injury(8).EHMT2 promotes neuron differentiation and creates a positive feedback loop that reinforces cellular commitment to differentiation(9). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Disease Association | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| EHMT2 is associated with retinal neurodegenerative diseases such as borderline glaucoma(10). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ChIP-Seq data
ChIP-Seq data of related histone modifications
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| Notice: For bigwiggle or bed file download, if the browser cannot automatically download the file, users can right click the hyperlink and then click the "Save link as..." button, which will automatically redirect to download dialogue box, or click the "Copy link address" button and access the website in a new tab page. For data analysis in Cistrome, users should first login into the Cistrome website, then press the "Send Bed" or "Send Big wiggle" button. For data download by Cistrome, users can follow this tutorial. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
References | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 1.Milner, C.M., and Campbell, R.D. (1993). The G9a gene in the human major histocompatibility complex encodes a novel protein containing ankyrin-like repeats. Biochem J 290 ( Pt 3), 811-818. 2.Collins, R.E., Northrop, J.P., Horton, J.R., Lee, D.Y., Zhang, X., Stallcup, M.R., and Cheng, X. (2008). The ankyrin repeats of G9a and GLP histone methyltransferases are mono- and dimethyllysine binding modules. Nat Struct Mol Biol 15, 245-250. 3.Tachibana, M., Sugimoto, K., Fukushima, T., and Shinkai, Y. (2001). Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3. J Biol Chem 276, 25309-25317. 4.Yu, Y., Song, C., Zhang, Q., DiMaggio, P.A., Garcia, B.A., York, A., Carey, M.F., and Grunstein, M. (2012). Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA. Mol Cell 46, 7-17. 5.Wu, H., Min, J., Lunin, V.V., Antoshenko, T., Dombrovski, L., Zeng, H., Allali-Hassani, A., Campagna-Slater, V., Vedadi, M., Arrowsmith, C.H., et al. (2010). Structural biology of human H3K9 methyltransferases. PLoS One 5, e8570. 6.Ferry, L., Fournier, A., Tsusaka, T., Adelmant, G., Shimazu, T., Matano, S., Kirsh, O., Amouroux, R., Dohmae, N., Suzuki, T., et al. (2017). Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation. Mol Cell 67, 550-565 e555. 7.Wang, Y.F., Zhang, J., Su, Y., Shen, Y.Y., Jiang, D.X., Hou, Y.Y., Geng, M.Y., Ding, J., and Chen, Y. (2017). G9a regulates breast cancer growth by modulating iron homeostasis through the repression of ferroxidase hephaestin. Nat Commun 8, 274. 8.Laumet, G., Garriga, J., Chen, S.R., Zhang, Y., Li, D.P., Smith, T.M., Dong, Y., Jelinek, J., Cesaroni, M., Issa, J.P., et al. (2015). G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition. Nat Neurosci 18, 1746-1755. 9.Fiszbein, A., Giono, L.E., Quaglino, A., Berardino, B.G., Sigaut, L., von Bilderling, C., Schor, I.E., Steinberg, J.H., Rossi, M., Pietrasanta, L.I., et al. (2016). Alternative Splicing of G9a Regulates Neuronal Differentiation. Cell Rep 14, 2797-2808. 10.Rao, R.C., Tchedre, K.T., Malik, M.T., Coleman, N., Fang, Y., Marquez, V.E., and Chen, D.F. (2010). Dynamic patterns of histone lysine methylation in the developing retina. Invest Ophthalmol Vis Sci 51, 6784-6792. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Figure Gallery
About Chromatin Regulator
Chromatin Regulator Cistrome
is a unique database integrating curated information of CRs, CR ChIP-seq datasets, CR related HM ChIP-seq datasets, and analysis of the relationship between CRs and HMs ChIP-seq pairs in human and mouse.