CR Name:
Species:    Tissue or Cell: 

Chromatin Regulator

Alias

CBX3HECH;HP1-GAMMA;

External Links:

Wiki    GeneCards    NCBI    UniProt

Related histone modifications:

H3K9me1;H3K9me2;H3K9me3

Introduction

Full name: Chromobox protein homolog 3. Cbx3 is a member of the family of heterochromatin protein 1 (HP1) proteins,which is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins and has been reported to specifically recognize methylated histone H3K9 mark.Cbx3 contains an N-terminal chromodomain (CHD) and a C-terminal dimerization chromo shadow domain (CSD) separated by a poorly conserved hinge region(1-3).

Function and Interaction

Cbx3 is a paralogue of heterochromatin protein 1 (HP1) proteins,posseses two conserved domains,the N-terminal chromodomain (CHD) which selectively recognizes methylated histone H3K9 mark,and the chromoshadow domain (CSD) through which HP1 forms dimers with other HP1 molecules or with other proteins and regulates the binding activity of the CHD(4-5).Cbx3 is involved in silencing at E2F- and MYC-responsive genes in G0 cells(6).Cbx3 is required for the repression of the progestin responsive MMTV promoter in breast cancer cells(7).In conjunction with SUV39H1,Cbx3 is also responsible for chromatin-mediated repression of the HIV-1 gene(8).

Disease Association

NA

ChIP-Seq data


SpeciesCell lineCell typeTissueDataDownloadSend to CistromeAnalysis FiguresComparisonReference
Homo sapiensK562ErythroblastBone MarrowGSE29611 ,GSM1003568
Click  DownloadClick22955616

ChIP-Seq data of related histone modifications


Products/SubstratesSpeciesCell lineCell typeTissueDataDownload Send to CistromeAnalysis FiguresReference
H3K9me2 Mus musculus None Embryonic Stem Cell Embryo GSE43231 ,GSM1059002 Bed     Big wiggle
Click   Download24361252
H3K9me3 Mus musculus None Embryonic Stem Cell Embryo GSE48519 ,GSM1180201 Bed     Big wiggle
Click   Download25263596
H3K9me3 Mus musculus None Embryonic Stem Cell Embryo GSE54947 ,GSM1327148 Bed     Big wiggle
Click   Download24735877
H3K9me3 Mus musculus None Embryonic Stem Cell Embryo GSE54947 ,GSM1327150 Bed     Big wiggle
Click   Download24735877
H3K9me2 Homo sapiens HCT-116 Epithelium Colon GSE58534 ,GSM1413015 Bed     Big wiggle
Click   Download26321255
H3K9me3 Homo sapiens HCT-116 Epithelium Colon GSE58638 ,GSM1420156 Bed     Big wiggle
Click   Download25747664
H3K9me3 Mus musculus E14 Embryonic Stem Cell Embryo GSE17642 ,GSM440257 Bed     Big wiggle
Click   Download19884257
H3K9me3 Mus musculus V6.5 Embryonic Stem Cell Embryo GSE18371 ,GSM459278 Bed     Big wiggle
Click   Download19884255
H3K9me3 Mus musculus V6.5 Embryonic Stem Cell Embryo GSE18371 ,GSM459281 Bed     Big wiggle
Click   Download19884255
H3K9me3 Mus musculus V6.5 Embryonic Stem Cell Embryo GSE18371 ,GSM459282 Bed     Big wiggle
Click   Download19884255
H3K9me3 Homo sapiens K562 Erythroblast Bone Marrow GSE28162 ,GSM607493 Bed     Big wiggle
Click   Download21596310
H3K9me3 Homo sapiens K562 Erythroblast Bone Marrow GSE24632 ,GSM607494 Bed     Big wiggle
Click   Download21170338
H3K9me3 Mus musculus J1 Embryonic Stem Cell Embryo GSE29413 ,GSM727425 Bed     Big wiggle
Click   Download21624812
H3K9me3 Mus musculus J1 Embryonic Stem Cell Embryo GSE29413 ,GSM727426 Bed     Big wiggle
Click   Download21624812
H3K9me3 Mus musculus J1 Embryonic Stem Cell Embryo GSE29413 ,GSM727429 Bed     Big wiggle
Click   Download21624812
H3K9me3 Homo sapiens K562 Erythroblast Bone Marrow GSE29611 ,GSM733776 Bed     Big wiggle
Click   Download22955616
H3K9me3 Mus musculus TNGA Embryonic Stem Cell Embryo GSE23943 ,GSM850405 Bed     Big wiggle
Click   Download22541430
H3K9me3 Mus musculus E14 Embryonic Stem Cell Embryo GSE23943 ,GSM850407 Bed     Big wiggle
Click   Download22541430
H3K9me3 Mus musculus E14 Embryonic Stem Cell Embryo GSE36114 ,GSM881363 Bed     Big wiggle
Click   Download22682255

Notice: For bigwiggle or bed file download, if the browser cannot automatically download the file, users can right click the hyperlink and then click the "Save link as..." button, which will automatically redirect to download dialogue box, or click the "Copy link address" button and access the website in a new tab page. For data analysis in Cistrome, users should first login into the Cistrome website, then press the "Send Bed" or "Send Big wiggle" button. For data download by Cistrome, users can follow this tutorial.

References

1.Eissenberg, J.C., and Elgin, S.C. (2000). The HP1 protein family: getting a grip on chromatin. Curr Opin Genet Dev 10, 204-210.
2.Nielsen, A.L., Oulad-Abdelghani, M., Ortiz, J.A., Remboutsika, E., Chambon, P., and Losson, R. (2001). Heterochromatin formation in mammalian cells: interaction between histones and HP1 proteins. Mol Cell 7, 729-739.
3.Ruan, J., Ouyang, H., Amaya, M.F., Ravichandran, M., Loppnau, P., Min, J., and Zang, J. (2012). Structural basis of the chromodomain of Cbx3 bound to methylated peptides from histone h1 and G9a. PLoS One 7, e35376.
4.Mishima, Y., Jayasinghe, C.D., Lu, K., Otani, J., Shirakawa, M., Kawakami, T., Kimura, H., Hojo, H., Carlton, P., Tajima, S., et al. (2015). Nucleosome compaction facilitates HP1gamma binding to methylated H3K9. Nucleic Acids Res 43, 10200-10212.
5.Lomberk, G., Wallrath, L., and Urrutia, R. (2006). The Heterochromatin Protein 1 family. Genome Biol 7, 228.
6.Ogawa, H., Ishiguro, K., Gaubatz, S., Livingston, D.M., and Nakatani, Y. (2002). A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells. Science 296, 1132-1136.
7.Vicent, G.P., Ballare, C., Nacht, A.S., Clausell, J., Subtil-Rodriguez, A., Quiles, I., Jordan, A., and Beato, M. (2006). Induction of progesterone target genes requires activation of Erk and Msk kinases and phosphorylation of histone H3. Mol Cell 24, 367-381.
8.du Chene, I., Basyuk, E., Lin, Y.L., Triboulet, R., Knezevich, A., Chable-Bessia, C., Mettling, C., Baillat, V., Reynes, J., Corbeau, P., et al. (2007). Suv39H1 and HP1gamma are responsible for chromatin-mediated HIV-1 transcriptional silencing and post-integration latency. EMBO J 26, 424-435.

Figure Gallery

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About Chromatin Regulator

Chromatin Regulator Cistrome
is a unique database integrating curated information of CRs, CR ChIP-seq datasets, CR related HM ChIP-seq datasets, and analysis of the relationship between CRs and HMs ChIP-seq pairs in human and mouse.