Chromatin Regulator | Alias | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PHF19 | PCL3;TDRD19B;MTF2L1;HPCL3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
External Links: | Wiki GeneCards NCBI UniProt | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Related histone modifications: | H3K36me2;H3K36me3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Introduction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Full name: PHD Finger Protein 19. PHF19 contains two isoforms,the long isoform contains an N-terminal TUDOR domain and two PHDs(plant homeodomains) while the short isoform lacks the second PHD finger(PHD2)(1).PHF19 associates with core subunits EZH2,SUZ12 and EED of PRC2,is a component of PRC2(1-5).PHF19 binds trimethylated histone H3 Lys36(H3K36me3) via its TUDOR domain and associates with H3K36me3 demethylase NO66,which is required for recruit the PRC2 complex and NO66 to stem cell genes during differentiation that mediates H3K27me3 methylation,followed by de novo silencing(2-4). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Function and Interaction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PHF19 has a pivotal role in silencing active promoters by directly targeting the PRC2 complex and H3K36 histone demethylases,is required for mES cell differentiation(2-4).PHF19 is a critical regulator of gene repression and mES cell self-renewal that acts by controlling PRC2 binding to target genes,particularly CpG islands regulated by PHF19(6).PHF19 plays an important role in the phenotype switch of melanoma cells from proliferative to invasive states(7). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Disease Association | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| NA | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ChIP-Seq data
ChIP-Seq data of related histone modifications
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| Notice: For bigwiggle or bed file download, if the browser cannot automatically download the file, users can right click the hyperlink and then click the "Save link as..." button, which will automatically redirect to download dialogue box, or click the "Copy link address" button and access the website in a new tab page. For data analysis in Cistrome, users should first login into the Cistrome website, then press the "Send Bed" or "Send Big wiggle" button. For data download by Cistrome, users can follow this tutorial. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
References | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 1.Boulay, G., Rosnoblet, C., Guerardel, C., Angrand, P.O., and Leprince, D. (2011). Functional characterization of human Polycomb-like 3 isoforms identifies them as components of distinct EZH2 protein complexes. Biochem J 434, 333-342. 2.Ballare, C., Lange, M., Lapinaite, A., Martin, G.M., Morey, L., Pascual, G., Liefke, R., Simon, B., Shi, Y., Gozani, O., et al. (2012). Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity. Nat Struct Mol Biol 19, 1257-1265. 3.Brien, G.L., Gambero, G., O'Connell, D.J., Jerman, E., Turner, S.A., Egan, C.M., Dunne, E.J., Jurgens, M.C., Wynne, K., Piao, L., et al. (2012). Polycomb PHF19 binds H3K36me3 and recruits PRC2 and demethylase NO66 to embryonic stem cell genes during differentiation. Nat Struct Mol Biol 19, 1273-1281. 4.Cai, L., Rothbart, S.B., Lu, R., Xu, B., Chen, W.Y., Tripathy, A., Rockowitz, S., Zheng, D., Patel, D.J., Allis, C.D., et al. (2013). An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting. Mol Cell 49, 571-582. 5.Wang, S., Robertson, G.P., and Zhu, J. (2004). A novel human homologue of Drosophila polycomblike gene is up-regulated in multiple cancers. Gene 343, 69-78. 6.Hunkapiller, J., Shen, Y., Diaz, A., Cagney, G., McCleary, D., Ramalho-Santos, M., Krogan, N., Ren, B., Song, J.S., and Reiter, J.F. (2012). Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal. PLoS Genet 8, e1002576. 7.Ghislin, S., Deshayes, F., Middendorp, S., Boggetto, N., and Alcaide-Loridan, C. (2012). PHF19 and Akt control the switch between proliferative and invasive states in melanoma. Cell Cycle 11, 1634-1645. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Figure Gallery
About Chromatin Regulator
Chromatin Regulator Cistrome
is a unique database integrating curated information of CRs, CR ChIP-seq datasets, CR related HM ChIP-seq datasets, and analysis of the relationship between CRs and HMs ChIP-seq pairs in human and mouse.